Functional expression of a Bombyx mori cocoonase: potential application for silk degumming

Processing of Bombyx mori silk for biomedical applications

This chapter highlights various processing methodologies that may be utilized to create a variety of structural forms from the protein fi broin, which is derived from the Bombyx mori silkworm cocoon. Modulation of fi broin material properties will be considered with respect to induced protein secondary structure formation. Additional considerations will be explored for sericin removal and fi br...

PTTH--a potential growth activator in silkworm, Bombyx mori L. for enhancing silk production.

In silkworm, prothoracicotropic hormone (PTTH), directly or indirectly controls silk production and spinning activity along with juvenile hormone (JH). An effort was made to exploit the potential of PTTH by indirectly activating silk gland for increasing silk productivity using short chain synthetic analogues of PTTH. The analogy in action was also established using PTTH extracted from the silk...

Expression of Cocoonase in Silkworm (Bombyx mori) Cells by Using a Recombinant Baculovirus and Its Bioactivity Assay

Conformation transition kinetics of Bombyx mori silk protein.

Time-resolved FTIR analysis was used to monitor the conformation transition induced by treating regenerated Bombyx mori silk fibroin films and solutions with different concentrations of ethanol. The resulting curves showing the kinetics of the transition for both films and fibroin solutions were influenced by the ethanol concentration. In addition, for silk fibroin solutions the protein concent...

Copper in the silk formation process of Bombyx mori silkworm.

Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis demonstrated that cupric ions formed coordination complexes with silk fibroin cha...